Analysis of flavonoids by bb-cyclodextrin modified capillary zone electrophoresis with field amplifi

来源 :第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会 | 被引量 : 0次 | 上传用户:juejue_wang11
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  Flavonoids occur as a group of active constutents in many traiditional Chinese medicines, so to analyze them accurately is of much importance.With the advantages of low consumption of reagents and samples, rapidity, and high resolution, capillary electrophoresis has has been widely appllied in flavonoids analysis.However, the short optical path length associated with on-column UV detection imposes an inherent detection problem for capillary electrophoresis.To address this problem, on-line extaction, coupling of capillary isotachophoresis with capillary zone electrophoresis and to extend the optical length by processing the capillary were developed.But all these above methods either involves complex operation or their sensitivity enhancements effects are often limited, which hinders their application.Most of the on-line concentration methods are based on changes in analyte migration due to differences between the sample and electrolyte conductivity or pH or through association with moving additives such as surfactants, and various combination of these effects.These methods enjoyed the advantages of simplicity and easiness for automation and have been developed to enhance the sensitivity for capillary electrophoresis.
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