新化合物GA-13315抑制肿瘤细胞DNA拓扑异构酶的作用及机制

来源 :2013医学前沿论坛暨第十三届全国肿瘤药理与化疗学术会议 | 被引量 : 0次 | 上传用户：peterwei2009

【摘要】

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【作者】

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张延辉陈亚娟陈静波张洪彬卿晨

【机构】

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昆明医科大学药学院/云南省天然药物药理重点实验室,云南昆明,650031 云南大学化学与工程学院/

【出处】

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2013医学前沿论坛暨第十三届全国肿瘤药理与化疗学术会议

【发表日期】

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2013年12期

【关键词】

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化合物GA-13315 抗肿瘤 DNA拓扑异构酶 DNA损伤

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目的研究新四环二萜类化合物GA-13315(以下简称GA)的抗肿瘤活性,并探讨其对肿瘤细胞DNA拓扑异构酶的作用及机制.方法以人胃腺癌细胞株SGC-7901(低分化)和MKN-28(高分化),人结肠腺癌细胞株LOVO(未分化)和HCr-116(低分化),人低分化肺腺癌细胞株GLC-82和AGZY-83a人肝细胞癌SMMC-7721(低分化)和HepG-2(高分化)为模型,采用MTT法检测化合物GA对多种人肿瘤细胞生长增殖的影响;通过以质粒DNA PBR322为底物的DNA解螺旋实验,检测化合物GA对人肿瘤细胞拓扑异构酶(TOPO)Ⅰ型和Ⅱ型催化活性的影响.

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新化合物GA-13315抑制肿瘤细胞DNA拓扑异构酶的作用及机制

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