Ribosome biogenesis is a basal cellular process in eukaryotic cells.Significant progress has been made on the biochemistry of ribosome biogenesis,however little is known about the cellular consequences due to impaired ribosome biogenesis.In this study,we cloned a small seed mutant dek*,and found it encodes maize Ribosome export associated 1 (ZmReal).Real is an AAA-ATPase in charge of 60S ribosome exportation from unclear to cytosol.The dek* appeared to be a weak mutant allele with decreased Real function.In dek* cells,mature 60S ribosome subunits are reduced both in nuclear and cytosol.However,the proportion of actively translating poly-ribosome is significantly increased in dek* cells.Reduced phosphorylation of eIF2α and increased protein level of eEFlα indicated the enhancement of general translational efficiency in dek* cells.Transcriptome and translatome analysis revealed dramatic difference between differently expressed genes (DEGs) and differently translated genes (DTGs),indicating significantly diverged responses at transcription and translation levels to the stress of ribosome shortage.Cell cycle transition is inhibited in the S phase in dek* cells,mediating slower growth.We built a model for reduced 60S ribosome exportation inconsistently regulating the transcription and translation of distinct groups of genes that affects general translation efficiency and cell cycle transition.