【摘 要】
：
Highly sensitive separations of biogenic compounds by capillary electrophoresis (CE) and microchip electrophoresis (MCE) using an on-line sample concentrati
【机 构】
：
DepartmentofMaterialChemistry,GraduateSchoolofEngineering,KyotoUniversity,Katsura,Nishikyo-ku,Kyoto6
【出 处】
：
第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
论文部分内容阅读
Highly sensitive separations of biogenic compounds by capillary electrophoresis (CE) and microchip electrophoresis (MCE) using an on-line sample concentration technique, i.e., large-volume sample stacking with an electroosmotic flow (EOF) pump or LVSEP [1,2], were investigated.In the LVSEP-MCE analysis of oligosaccharides released from bovine ribonuclease B, more than 2000-fold improvement of the detectability was achieved compared to a conventional MCE analysis using the pinched-injection.In LVSEP-CE of oligosaccharides, ca.780-fold sensitivity enhancement was attained without any loss of resolution compared to a conventional CE analysis.To make further sensitivity improvement possible, LVSEP was combined with the field-amplified sample injection (FASI) technique.Maltoheptaose (G7) derivatized with 9-aminopyrene-1,4,6-trisulfonic acid (APTS) was analyzed by LVSEP-FASI-CE.As a typical result, G7 was well concentrated and separated from the free APTS with a 10,000-fold sensitivity increase compared to the conventional CE analysis, where the good repeatability of the migration time (%RSD =2.4; n =3) was obtained.
其他文献
Applications of chromatography technologies have become more and more important as the basic chromatography theory and technologies have been treading to ma
From a traditional definition in the field of human health, metabolomics measures the concentrations of the large number of naturally occurring small molecu
Dan Shen (Rdix salviae miltiorrhizae) is one of the most commonly used Chinese herbs.It could regulate blood circulation and address various disorders cause
Graphene, with 2D structure of single-atom-thick sheet of packed carbon (sp2 hybridized) atoms, possesses numerous fascinating properties such as large surf
A simple, rapid capillary electrophoresis-laser-induced fluorescence (CE-LIF) method for the assay of recombinant human erythropoietin (rhEPO) was developed
In recent years, hollow fiber based liquid-liquid-liquid micro-extraction (HF-LLLME) [1] has been widely studied due to its higher enrichment factor, excell
The development of new biomarkers to improve the diagnosis, prognosis and management of chronic obstructive pulmonary disease (COPD) is an urgent medical ne
A novel on-line two-dimensional system was developed for peptide and protein mapping.The system combines capillary isoelectric focusing (cIEF) with pressuri
Surface plasmon resonance imaging (SPRi) [1-3] is a new, labeling-free and high-throughput analytical tool capable of imitating biological conditions and co
A novel purge-assisted and temperature-controlled headspace solid-phase microextraction (PATC-HS-SPME) couple to gas chromatography-negative ion chemical io