Wnt/β-catenin signaling is essential for embryogenesis and tissue homeostasis,which has been highly conserved through evolution.Deregulation of Wnt/β-catenin signals can initiate and promote human cancers,specifically of colon and head and neck.As such,Wnt/β-catenin signaling represents an attractive target for cancer therapy.The interaction between β-catenin and the transcription factor TCF4 is necessary in the transduction of the signal.We performed High Throughput Screening (HTS) using AlphaScreen and ELISA techniquesto identify small molecules that disrupt this interaction.