Ferroportin1 (FP1) and hephaestin (HP) are newly-found iron transporters cooperating in the iron effiux process in the gut.While their roles in brain iron homeostasis are not fully elucidated.Our previous study demonstrated that decreased FP1 and HP expression were involved in the iron accumulation and dopaminergic neurons degeneration in Parkinsons disease (PD) animal models.To investigate whether overexpressed FP1 and/or HP could attenuate iron induced oxidative stress,in the present study we generated the stable MES23.5 dopaminergic cell lines with over expression of FP1 and/or HP,and the dopamine (DA) content and cellular oxidative stress under iron overloaded circumstances in control and transfected cells were observed.The results showed that iron reduced DA content in the control,however,a significantly less reduction of DA content was observed in transfected cells with high expression of FP1 and/or HP.