【摘 要】
：
Objective The non-coding miRNAs are processed in a step-wise manner from the genomic DNA sequences through pri-miRNAs and pre-miRNAs to the matured 21-25bp nucleotides(usually 22bp),during which cours
【机 构】
：
Institute of Comparative Medicine & Center of Experimental Animals,Southern Medical University,Guang
论文部分内容阅读
Objective The non-coding miRNAs are processed in a step-wise manner from the genomic DNA sequences through pri-miRNAs and pre-miRNAs to the matured 21-25bp nucleotides(usually 22bp),during which course three enzymes referred to as Pasha,Drosha and Dicer(Pasha and Drosha exist in the nucleus while Dicer in the cytoplasm)play the key roles for the miRNA biogenesis.Therefore,the deletion of each of the above enzymes will block the miRNA biogenesis pathway,causing the down expression of most miRNAs and resulting in the animal embryonic defect.However,it is surprisingly found that the knocked out animals of Pasha,Drosha and Dicer have different phenotypes from one another,indicating the three enzymes may have their respectively additional roles in animals.Till now several tissue-specific conditional miRNA biogenesis enzyme knockout mice have been established to determine the roles of the enzymatic genes in different tissues,which further expounds the related miRNA functions in whole animal bodies.
其他文献
In this study,a strain with the function of desulfurization and denitrification,T1,was isolated from EGSB-DSR reactor.In metabolic of T1,sulfide was adopted for its electron donor and nitrate for elec
Wildfires are important disturbances that help to shape the structure and function of forest and grassland ecosystems.The impacts of fire on aboveground community composition and diversity have been e
猪繁殖与呼吸综合征病毒(PRRSV)能够导致母猪繁殖性能下降、不孕、流产等,在猪场中往往与其他病原混合感染,给该病毒的诊断带来较大困难.本实验设计合成了一套引物和TaqMan探针,以扩增PRRSV的核衣壳蛋白(N)基因,通过反应条件的优化,建立了快速定量检测PRRSV的荧光定量RT-PCR方法.结果表明该方法具有较高的特异性、重复性和敏感性,对PRRSV细胞培养物的检测下限为0.01 ×TCID5
目的 实验动物质量决定了实验动物标准化程度，对动物实验的结果起着关键作用，包括微生物和寄生虫、遗传学、营养学和环境生态学。本文结合笔者认识及工作实践，就动物使用过程中实验动物质量的控制，从管理方面进行初步探讨。
Objective Alzheimers disease(AD)is the most common form of dementia.With increased life expectancy,this number is expected torise in the future.AD is characterized by progressive memorydeficits,cognit
目的 本研究旨在研究不同饲料硬度对实验大小鼠的生长性能、饲料采食量和耗损量的影响.选用60只实验小鼠随机分为3个处理组，雌雄各半，分别饲喂硬度梯度为9.50、8.34和7.07的日粮，试验期45天，结果表明：随着日粮硬度梯度的提高，实验小鼠无论雌雄，其采食量、饲料耗损量均显著下降(P＜o.05)，采食量与耗损量比值显著增加(P＜0.05)，雄性实验小鼠总蛋白、球蛋白、天门冬氨酸转氨酶、血清胆固醇和
Objective Inflammatory bowel diseases(IBDs,two major types being ulcerative colitis and crohns disease)are representative chronic inflammatory conditions of the colon and can increase the risk of deve
Objective In Thailand,trypanosomiasis or surra outbreaks in pigs has been reported throughout Thailand with varying clinical manifestations.Therefore,the aim of this study is to evaluate levels of pro
Objective The strategy to investigate an individual miRNA function in vivo is to delete or to overexpress it in animals.It is demonstrated that individual microRNAs can exert critical control over mam