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Anglotensin Ⅱ (Ang Ⅱ) plays a key role in the regulation of blood pressure and the process of cerebral ischemia.We have previously demonstrated that pinocembrin (5,7-dihydroxyflavanone), one of the main flavonoids in propolis, improves cerebral blood flow in focal or global cerebral ischemia-reperfusion rats.Here, we investigate the vasorelaxant effect of pinocembrin on Ang Ⅱ-induced vasocontraction and its molecular mechanism of action.We show that pretreatment with pinocembrin inhibited Ang Ⅱ-induced vascular contractions in both endothelium-intact and endothelium-denuded rat aortic rings.This inhibition was associated with a significant attenuation of the myosin light chain 2 phosphorylation at Ser19 that result from Ang lⅡ stimulation.In rat vascular smooth muscle cells, pinocembrin significantly suppressed Ang Ⅱ-induced Ca2+ influx.Taken together, our results demonstrate that pinocembrin inhibits Ang Ⅱ-induced rat aortic rings contraction, most likely through the inhibition of Ang Ⅱ-induced Ca2+ influx.