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Chinese fir is one of the most important commercial tree species in China.Nowadays, frequent seasonal drought has the serious negative effects on Chinese fir production.In this study, under PEG-6000-stimulated drought stress, using FS43 (a drought-tolerant Chinese fir clone) and FS38 (a drought-sensitive Chinese fir clone), we investigated the changes in the Chinese fir leaf proteome in response to drought stress.