Biodegradation of ferulic acid by a newly isolated strain of Cupriavidus sp.B-8

来源 :第八届全国青年岩土力学与工程会议暨青年华人岩土工程论坛 | 被引量 : 0次 | 上传用户:lostlovestefan
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  As a major component of lignin and abundantly existing in softwood and hardwood, ferulic acid has been used as a lignin-related compound for lignin biodegradation study.Biodegradation of ferulic acid by Cupriavidus sp.B-8, a newly isolated strain, was studied.This strain is able to utilize a wide range of lignin-related aromatic compounds as the sole carbon and energy source, including guaiacol, veratric acid, vanillic acid, cinnamic acid, p-coumaric acid, ferulic acid, and sinapic acid.In addition, the effects of different concentrations of ferulic acid on growth of Cupriavidus sp.B-8 were studied.The growth of Cupriavidus sp.B-8 is better under the condition of lower concentration.High-performance liquid chromatography (HPLC) analysis reveals that above 95% of ferulic acid is degraded within 12 h by Cupriavidus sp.B-8.Based on identification of biodegradation intermediates and further metabolites, the biodegradation pathway of ferulic acid by Cupriavidus sp.B-8 was proposed.Ferulic acid is initially converted to 4-vinylguaiacol, and further oxidized to vanillic acid and protocatechuic acid.
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