MiR-17~92对胰岛β细胞功能的调控

来源 :中华医学会第十四次全国内分泌学学术会议 | 被引量 : 0次 | 上传用户:qq11202365
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目的 明确miR-17~92在胰岛β细胞功能调控中的作用以及机理方法 1)通过Cre-loxp系统构建胰岛β细胞特异性敲除miR-17~92基因的基因工程鼠;常规监测小鼠代谢相关指标;测量基因敲除鼠在体和离体的胰岛素分泌情况;观察胰腺的组织形态学并定量计算β细胞数量、体积、质量,胰岛β细胞的凋亡、增殖能力;荧光定量RT-PCR检测胰岛细胞胰岛素合成分泌相关基因表达情况.Western实验检测PTEN表达量变化.2)小鼠8周龄开始高脂诱导至24周龄.高脂诱导期间常规测量四组小鼠体重、空腹血糖、随机血糖和血脂等指标.测量四组小鼠在体和离体的胰岛素分泌情况.胰腺行组织病理学分析,观察胰岛β细胞总量及凋亡、增殖水平的变化.
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