2. 您的位置
3. 首页
4. 会议论文
5. 转录因子FOXO4与大鼠软骨细胞退变的相关性研究

转录因子FOXO4与大鼠软骨细胞退变的相关性研究

来源 :中国生物化学与分子生物学会第十二届会员代表大会暨2018年全国学术会议 | 被引量 : 0次 | 上传用户：safafaffaf

【摘 要】

：

目的：研究转录因子FOXO4与大鼠软骨细胞退变的相关性。方法：取4周和16周SD大鼠的软骨，分离软骨细胞；使用甲苯胺蓝染色、阿利新蓝染色及Ⅱ型胶原蛋白免疫细胞化学染色等方法进行软骨细胞鉴定；以1L-1β对大鼠软骨细胞进行诱导退变处理；镜下观察和采用β-半乳糖苷酶染色法进行衰老检测；使用PCR和WesternBlot方法分别检测软骨细胞中FOXO4和Col2α的mRNA表达水平和蛋白表达水平。

【作 者】

：

余文敏 殷嫦嫦 周许峰 张义平 曹俊 王丽丽 陈川 吴伟 

【机 构】

：

九江学院基础医学院/九江市转化医学重点实验室 江西 九江 332000

【出 处】

：

中国生物化学与分子生物学会第十二届会员代表大会暨2018年全国学术会议

【发表日期】

：

2018年10期

【关键词】

：

骨性关节炎 软骨细胞 退变模型 FOXO4 Ⅱ型胶原蛋白 

下载到本地 , 更方便阅读

下载此文 赞助VIP

声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架

论文部分内容阅读

　　目的：研究转录因子FOXO4与大鼠软骨细胞退变的相关性。方法：取4周和16周SD大鼠的软骨，分离软骨细胞；使用甲苯胺蓝染色、阿利新蓝染色及Ⅱ型胶原蛋白免疫细胞化学染色等方法进行软骨细胞鉴定；以1L-1β对大鼠软骨细胞进行诱导退变处理；镜下观察和采用β-半乳糖苷酶染色法进行衰老检测；使用PCR和WesternBlot方法分别检测软骨细胞中FOXO4和Col2α的mRNA表达水平和蛋白表达水平。

其他文献

Regulation of LDL cholesterol by LIMA1/SREBP3

High levels of low-density-lipoprotein cholesterol(LDL-C)is a major risk factor for cardiovascular disease.Although LDL-C levels vary among humans and are heritable,the genetic factors affecting LDL-C

会议

cholesterolLIMA1LDLNPC1L1

Six1 promotes proliferation and migration of human colorectal cancer cells through activating Wnt/β-

Six1 is a homeodomain transcription factor that is aberrantly expressed in a variety of human cancers,including colorectal cancer(CRC).Six1 has been reported to play a key role in the proliferation an

会议

Six1colorectal cancerproliferationmigrationβ-catenin

NCAM modulates insulin signaling and adipocyte differentiation of mesenchymal stem cells via AMPK/p3

Neural cell adhesion molecule(NCAM)deficiency induces the expression of tumor necrosis factor-α(TNF-α),thereby leading to insulin resistance and impaired adipogenesis of mesenchymal stem cells(MSCs).H

会议

NCAMTNF-αAMPKinsulin signalingadipocyte differentiation

AZ/AZIN调控对A549非小细胞肺癌细胞作用研究

肿瘤的快速生长高度依赖于多胺，使得多胺的代谢途径成为抗肿瘤治疗研究的新靶点，特别是以鸟氨酸脱羧酶(ODC)为核心，鸟氨酸脱羧酶抗酶(AZ)和鸟氨酸脱羧酶抗酶抑制因子(AZIN)共同调节的调控网络。基于现有的针对于ODC抑制的抑制剂临床应用弊端，本课题拟探索以AZ/AZIN通路作为抗肿瘤治疗分子靶点的潜在价值。

会议

人非小细胞肺癌A549细胞鸟氨酸脱羧酶鸟氨酸脱羧酶抗酶鸟氨酸脱羧酶抗酶抑制因子

The ER Stress Response Signaling in Metabolism and Cancer

会议

Ursolic acid inhibits IL-1β-induced release of IL-6 and IL-8 via suppression of AKT,ERK and NF-kappa

Ursolic acid(Ursolic acid,UA),a pentacyclic triterpenoid compound present in a wide variety of plant foods,is reported to have anti-inflammatory,anti-tumor,anti-oxidant and anti-diabetes and other bio

会议

Ursolic acidSW982 cellsInflammatory factorsIL-1 betaSignal pathway

Structural basis of highly specific interaction between Nephrin and MAGI1 in slit diaphragm assembly

Slit diaphragm(SD)is a specialized adhesion junction between the opposing podocytes,establishing the final filtration barrier to prevent passage of proteins from the capillary lumen into the urinary s

会议

nephrinnephrotic syndromeglomerular filtration barriercell adhesion

Mild hypothermia prevents NO-induced cytotoxicity in human neuroblastoma cells via COX-2 induction

Mild hypothermia and cold-inducible protein RBM3 can protect neural cells from nitric oxide(NO)-induced apoptosis.Meanwhile,it is known that cyclooxygenase(COX)-2 is upregulated by cold-inducible prot

会议

mild hypothermiaRBM3COX-2neuroprotectionnitric oxide

Changes in prothrombin time and platelet parameters of mice treated with vitamins C and E

This study aimed to elucidate the effects of supplementation with different doses of vitamin C(VC)and vitamin E(VE)on prothrombin time(PT)in mice; Forty-eight mice were randomly divided into six treat

会议

Vitamin CVitamin EProthrombin timePlatelet parameters

Arsenic trioxide combined with miR-330-5p synergistically enhances pancreatic cancer cell proliferat

Arsenic trioxide(ATO)has been found to exert anti-cancer activity in various human malignancies.In our previously reports,we have reported that ATO could inhibits cell growth and invasion via down-reg

会议

arsenic trioxideSkp2miRNA-330-5ppancreatic cancer