【摘 要】
：
Flavonoids are the main components of Meconopsis integrifolia (Maxim.) Franch, which is a traditional Tibetan medicine.However, traditional chromatography separation requires large quantity of raw M.i
【机 构】
：
Yao College of Medical, Guangxi University of Chinese Medicine, Nanning, Guangxi, China;Institute of
【出 处】
：
2016首届中国中药资源大会暨CSNR中药及天然药物资源研究专业委员会第十二届学术年会
论文部分内容阅读
Flavonoids are the main components of Meconopsis integrifolia (Maxim.) Franch, which is a traditional Tibetan medicine.However, traditional chromatography separation requires large quantity of raw M.integrifolia and is very time-consuming.Herein, we applied high-speed counter-current chromatography (HSCCC) in the separation and purification of flavonoids from the ethanol extracts of M.integrifolia flower.Ethylacetate/n-butanol/water (2:3:5, v/v/v) was selected as the optimum solvent system to purify the four components, namely quercetin-3-O-β-D-glucopyrannosy-(1→6)-β-D-glucopyranoside (compound 1, 60 mg), quercetin 3-O-[2"-O-acetyl-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside](compound 2, 40 mg), quercetin 3-O-[3"-O-acetyl-β-D-glucopyranosyl-(1→6)-β-D-Glucopyranoside] (compound 3, 11 mg) and quercetin 3-O-[6"-O-acetyl-β-D-glucopyranosyl -(l→6)-β-D-glucopyranoside] (compound 4, 16 mg).Among the four compounds, compound 3 and 4 were new discovered acetylated flavonol diglucosides.After the HSCCC separation, the purities of the four flavonol diglucosides were 98%, 95%, 90% and 92%, respectively.The structures of them were identified by MS and NMR spectra.
其他文献
目的：建立同时测定野罂粟碱、黑龙辛甲醚及瑞芙热米定三种主要生物碱含量及快速检测野罂粟总生物碱含量的方法,并对不同居群野罂粟进行测定；此外,以生物碱类成分的含量为指标,进行聚类分析。方法：采用超声辅助法提取样品,外标法定量,采用LCMS-IT-TOF法对野罂粟全草进行定性分析,采用溴甲酚绿酸性染料比色法测定野罂粟的总生物碱含量,采用UPLC-MS法测定野罂粟碱、黑龙辛甲醚及瑞芙热米定三种主要生物碱含
目的：对引起栀子炭疽病的病原菌茎点霉菌Phoma.sp抑菌药剂进行室内筛选试验,研究常用杀菌剂对病原菌菌丝生长的抑制作用,为病害防治提供依据.方法：选取常用的11种抑菌剂对其进行室内药剂毒力测定,并建立抑制率与药剂浓度的相关回归方程；筛选出有明显抑菌作用的药剂,为防治该病的发生提供理论依据.结果：选用的药剂中武夷菌素、链霉素和寡糖链蛋白无明显的抑菌效果,多菌灵的抑菌效果最好,回归方程为Y=1.84
BAG蛋白能够作为分子伴侣调节蛋白广泛的参与动物细胞内多种进程的调节,包括细胞的增殖、生长以及凋亡途径.拟南芥BAG蛋白(Arabidopsis thaliana BAG protein,AtBAG protein)家族共有七个成员,它们主要分布在一号、二号、三号及五号染色体上,其中五号染色体上分布着四个成员,其他染色体上无基因聚类.根据其结构组成的差异可以分为两组.第一组由AtBAG l-4组成
人参是东北重要的道地性药材,人参皂苷为其主要药效成分,人参皂苷的生物合成受到环境因子和合成途径中关键酶基因的共同调控.由于人参栽培年限长、连作障碍、病虫害严重,很大程度影响了人参产量和质量.野生人参资源又非常稀少,天然资源受到限制,而传统的的田间种植栽培已经很难满足日益增长的人参市场需求,导致人参资源短缺.为进一步揭示人参皂苷生物合成的生理生态机制,本研究利用分子生物技术手段对人参皂苷生物合成关键
目的：研究地榆种子的抗旱耐盐性,明确干旱胁迫与盐胁迫对其种子萌发的影响.方法：利用不同浓度的PEG溶液模拟干旱条件,不同浓度的Nacl以及Na2SO4溶液模拟盐胁迫条件,测定种子萌发率等指数.结果：适度的干旱可以促进种子萌发并提高萌发指数,低浓度的Nacl可以促进地榆种子的萌发,高浓度抑制,Na2SO4溶对地榆种子萌发没有促进作用,抑制作用大于Nacl.
在9个不同变异类型铁皮石斛中,TP5型多糖含量(45.14％)、TP6型甘露糖含量(30.42％)、 TP7型蛋白质含量(1.12 g·100g-1)和TP1型总氨基酸含量(34.15 mg·g-1)最高,而TP9型粗纤维含量最低(3.37％).从生长年限而言,二年生铁皮石斛茎的折干率、多糖、甘露糖、粗纤维、蛋白质和总氨基酸含量等指标均高于三年生铁皮石斛.该研究结果对铁皮石斛种质资源的综合可持续利
Pseudostellaria heterophylla is an important traditional Chinese herbal medicine with vast clinical consumption because of its positive effects.To date, changes in the metabolite composition of Pseudo
目的：研究三七对不同类型水稻品种的化感作用，探讨三七和水稻水旱轮作可行性。方法：以不同类型水稻为受体，对三七茎叶、根水浸液及绒根的化感作用进行生物检测。结果：①三七茎叶、根水浸液及绒根对供试水稻均表现出明显的化感抑制作用。其中，茎叶比根水浸液抑制作用强，绒根随添加量增大，抑制作用不断加强；②旱稻旱丰100对三七化感作用敏感性最强，粳稻鄂粳杂l号次之，籼稻T优300最弱。结论：籼稻T优300可以考虑
Puerariae lobatae Radix, also known as gegen in China, is a commonly used traditional Chinese medicine for about 2000 years.Itis widely used in the pharmaceutical, food and cosmetic industries.For the
Background: The traditional Chinese medicine (TCM) Qianhu and Zihuaqianhu are the dried roots of Peucedanum praeruptorum and Angelica decursiva, respectively.Since the plant sources of Qianhu and Zihu