A semi-continuous solid state fermentation process using sugarcane bagasse(SCB)for production of sugars and bioethanol is introduced.Alkali-treated SCB supplemented with wheat bran(WB)was first subjected to mixed culture solid-state fermentation(MSSF)with Trichoderma and Penicillium to produce a compound enzyme,and after MSSF(about 72 h),the substrate mixture was directly fermented by inoculating Saccharomyces cerevisiae.Fungal inoculation and in situ produced enzymes resulted in a high level of sugars generation.The highest total and reducing sugars concentrations 20.190 g/L and 12.468 g/L were obtained respectively after 72 h culturing,?-glucosidase activity and fungal biomass produced were 0.483 IU/mL and 0.204 g/g respectively at 144 h.The maximum ethanol concentration 5.825 g/L(40.84%of theoretical yield)was achieved with fermentation of the MSSF substrate mixtures in the first 24 h.These results indicate that using semicontinuous MSSF may be an effective approach for the economic production of bioethanol without employing commercial cellulases.