Epithelial-mesenchymal transition (EMT) is an important factor in lung cancer metastasis,and targeting EMT is a potential therapeutic strategy.Fucosyltransferase Ⅳ (FUT4) and its synthetic cancer sugar antigen Lewis Y was abnormally elevated in many cancers.A traditional Chinese medicine ginsenoside Rg3 was used to investigate whether its inhibition to EMT and invasion of lung cancer is by glycobiology mechanism.We found that Rg3 inhibited cell migration and invasion by wound-healing and transwell assays.Rg3 significantly altered E-cadherin,Snail,N-cadherin and Vimentin expression.Rg3 down-regulated FUT4 expression in lung cancer cells by qPCR,Western blot and immunofluorescence.