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Objective To study the clinical characteristics and laboratory findings of Mycoplasma pneumoniae with EBV infection in children, and provide an experimental basis for clinical diagnosis and treatment.Methods Children hospitalized in respiratory department during May 2013 to April 2014 were enrolled.122 children with M.pneumoniae and EBV infection constituted experimental group, 45 cases children of M.Pneumoniae infection and 41 cases children of EBV infection formed control group.