【摘 要】
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Alternaria is a common genus of fungi,and contains numerous species that are both saprophytic on organic materials and pathogenic on many plants.The Alternaria genus produces more than 70 known mycoto
【机 构】
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Beijing Technology and Business University,Beijing Lab for Food Quality and Safety,Beijing 100048,Ch
【出 处】
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43rd International Symposium on High Performance Liquid Phas
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Alternaria is a common genus of fungi,and contains numerous species that are both saprophytic on organic materials and pathogenic on many plants.The Alternaria genus produces more than 70 known mycotoxins and the most important Alternaria mycotoxins are alternariol(AOH),tenuazonic acid(TeA),altenuene(ALT),alternariol monomethyl ether(AME),and altertoxins Ⅰ,Ⅱ,Ⅲ(ATX-Ⅰ,Ⅱ,Ⅲ)produced by Alternaria alternata [1].Alternaria mycotoxins are frequently found in variety of food at low level and have potential health risk for humans and animals.In all of the analytical cases,previous preconcentration/cleaning step to selectively separate this mycotoxin from complex matrices is necessary.In this paper,a new pretreatment method with countercurrent chromatography(CCC)was developed for enrichment and cleanup of minor Alternaria mycotoxins in liquid food samples prior to HPLC analysis.A two-phase solvent system composed of ethyl acetate and water containing 6%[HOOMIM]Cl in mass ratio was employed.The enrichment and cleanup of Alternaria mycotoxins can be fulfilled online by this method with high enrichment factors(87~114)and high recoveries(81.14~110.94%)under the optimized CCC pretreatment conditions.The method has been successively applied to the determination of Alternaria mycotoxins in real apple juice and wine samples with the limits of detection(LOD)in the range of 0.03~0.14μg/L.The proposed method(IL-CCC)is simple,rapid,and sensitive and could also be used for the analysis and monitoring of Alternaria mycotoxin levels in other food samples.
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