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Purpose: Effective prognostic biomarkers specific to advanced stages are urgently needed for better management of prostate cancer.Increased COL6A1 expression that correlates with the metastatic prostate cancer was studied for this purpose.Methods: COL6A1 expression pattern was determined by qRT-PCR and western blot analysis in all three prostate cancer cell lines(PC-3, DU145, and LNCaP).Using Transwell and wound healing assays, we determined the role of COL6A1 in cell invasion and migration,respectively.For clinical relevance, we determined COL6A1 protein levels in 223 localized (N=79) or metastatic prostate (N=144) cancertissues.Results: COL6A1 expression was higher in PC-3 and DU145 than LNCaP.It was a positive regulator of both cell invasion and migration and correlated with two known indicators of cell migration: MMP-9 and CXCR4.Furthermore, COL6A1 levels were significantly higher in advanced stage prostate cancer with both bone and/or lymph node metastasis than early stage prostate cancer.Overexpression of COL6A1 was associated with poor prognosis in metastatic prostate cancer patients.Conclusion: Increased COLgA1 expression may indicate advanced stage prostate cancer with bone and lymph node metastasis.