【摘 要】
：
Discrepancy between the in-vivo and in-vitro drug sensitivity patterns of human turnout cells has been reported many times.It is often observed that drug screening tests show major differences in in-v
【机 构】
：
Karolinska Institute Sweden
【出 处】
：
BIT Life Sciences 1st Annual World Cancer Congess-2008(2008中
论文部分内容阅读
Discrepancy between the in-vivo and in-vitro drug sensitivity patterns of human turnout cells has been reported many times.It is often observed that drug screening tests show major differences in in-vitro culture as compared to subsequent in-vivo studies or therapeutic results in patients.This is likely caused by differences in the environmental conditions in-vivo and in-vitro.Unlike cell lines, primary human tumours are particularly sensitive for oxidative stress which can be caused by exposing them to air during sample taking and culturing.Another important issue is the culturing media which is lacking the important growth factors as compared to the in-vivo environment.We aim to decrease this gap by using many new factors which simulates in vivo conditions as closely as possible.We have shown that our screening system is superior to support survival of primary tumours as well as to decrease the sensitivity of tumour cells to most commonly used cytotoxic drugs.We use this method to determine the drug sensitivity pattern of cancer patients with the aim to provide support for clinicians for assay guided individualized therapy.We have also identified some alternative treatment options for CLL, late stage Hodgkin disease and PTLD.Our system also allows survival assays in mixed cell culture systems e.g.fluorescence NK or CTL killing assays or tumorcell/stromal cell drug sensitivity assays.
其他文献
Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome which afflicts multiple organs and for which there is no cure, such that TSC patients may develop severe mental retardation and
Although cross-resistance between radiation and cis-platinum is well documented, both experimentally and clinically the molecular basis of these phenomena remains unknown.We have completed the rnicroa
Background: Collimator based, single fraction intraoperative radiotherapy (IORT) has recently been explored at a limited number of institutions as a potential replacement for conventional whole breast
While local tumor growth is managed relatively successfully by usual cancer treatment modalities (surgical excision, radio-and chemo-therapy), treatment of metastatic diseases too often fails.While ca
When a patients health care team determines that the cancer can no longer be controlled, medical testing and cancer treatment often stop.But the patients care continues.The care focuses on making the
Cancer is a leading health problem for elderly persons in Taiwan.Women of old age, whose opinions towards physical health are influenced by traditional values and cultural background.This study aimed
Phytonutrients such as carotenoids, polyphenols and isothiocyanates were found by us to inhibit sex steroid signaling, the major risk factor for breast cancer.However, estrogens are beneficial for bon
There has been an increase in the life span of cancer patients, thanks to the advances in diagnostic tools and treatment modalities.Most of these patients gain functional capacity and return to their
The lecture will demonstrate the collaboration and motivation of the interdisciplinary team members in making a dream come true and redefining the notion of successful palliative treatment.Our present
Ovarian cancer is the fifth most common cause of death from cancer in women.The standard first-line treatment is a combination of paclitaxel and carboplatin (DDP) or carboplatin alone.In the case of p