【摘 要】
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In order to determine the active compounds from Denis cavaleriei vines and their antifeedant activities against Plutella xylostella larvae,the active compounds were isolated by activity-guided fractio
【机 构】
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College of Bio-Safety Science and Technology,Hunan Agricultural University,Changsha 410128,China
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In order to determine the active compounds from Denis cavaleriei vines and their antifeedant activities against Plutella xylostella larvae,the active compounds were isolated by activity-guided fractionation with column chromatography and identified on the basis of MS and NMR data,and antifeedant activity was determined by sandwich method.The results showed that four compounds,isolonchcarpin (A),Magnificol (B),ovalifla,vanone (C) and millettocaiyxin C (D) had been isolated from the plant vines for the first time.Isolonchcarpin,Magnificol and millettocalyxin C had been exhibited antifeeding activity,with AFC50 values of 25.5 mg·L-1,92.8 mg·L-1 and 115.9 mg·L-1 against the third instar larvae of P.xylostella at 48 h after treatment,respectively.The above-mentioned three compounds were main antifeedant compounds derived from the plant vines against P.xylostella.The elucidation of these chemicals is important not only for understanding the insect-plant relationships,but also for their potential in P.xylostella control.
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