【摘 要】
：
目的:miR-291 b-3p是miR-290家族中的一员,在胚胎发育过程中起重要作用,而在脂代谢中的作用尚未见报道,本研究旨在阐明其在肝脏脂质代谢中的作用及其机制.方法:首先在NCTC 14
【机 构】
：
北京大学第五临床医学院(卫生部北京医院);卫生部北京老年医学研究所,卫生部老年医学重点实验室卫生部北京老年医学研究所,卫生部老年医学重点实验室;中国科学院生物物理研究所卫生部北京老年医学研究所,卫生部
【出 处】
：
2014年第十二届全国脂质与脂蛋白学术会议
论文部分内容阅读
目的:miR-291 b-3p是miR-290家族中的一员,在胚胎发育过程中起重要作用,而在脂代谢中的作用尚未见报道,本研究旨在阐明其在肝脏脂质代谢中的作用及其机制.方法:首先在NCTC 1469细胞内通过油酸/棕榈酸(2∶1)刺激24h造成细胞内脂质蓄积,油红O检测细胞内脂质蓄积情况,western blot检测脂代谢相关蛋白的表达,q-PCR检测miR-291b-3p在细胞内的表达.其次在细胞脂质蓄积模型内转染miR-291b-3p的inhibitor,油红O检测细胞内脂质蓄积情况,western blot检测脂代谢相关蛋白的表达.再次通过软件预测寻找miR-291b-3p潜在的靶基因,通过荧光素酶活力分析及western blot检测其与靶基因的关系.
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