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目的:探讨白头翁汤治疗炎症性肠病(IBD)的分子机制。方法:84只BALB/c小鼠随机分为7组(n=12):乙醇对照组、模型组、SASP组、白头翁汤组(低、中和高剂量组)和zVAD组。各组分别灌胃和腹腔给药。疗程结束后取小鼠结肠组织分别利用荧光定量RT-PCR、Elisa和免疫组化等方法检测NLRP3、ASC、Caspase-1、IL-18和IL-1β在结肠组织中的表达量。结果:与模型组比较,SASP组和中、高剂量白头翁汤对IBD有显著的治疗作用(P<0.01),但这种治疗作用在zVAD组被抑制(P<0.05)。同时,中、高剂量白头翁汤能够升高NLRP3、ASC、Caspase-1、IL-18和IL-1β在结肠组织的表达(P<0.05),而zVAD能抑制这种上调(P<0.05)。结论:白头翁汤可能通过激活NLRP3炎症小体进而促进相应的炎症因子包括IL-18及IL-1β的生成来发挥治疗IBD的作用。
Objective: To investigate the molecular mechanism of Pulsatilla Decoction in the treatment of inflammatory bowel disease (IBD). Methods: Eighty-four BALB / c mice were randomly divided into 7 groups (n = 12): ethanol control group, model group, SASP group, Pulsatilla Decoction group (low, middle and high dose) and zVAD group. Groups were intragastric and intraperitoneal administration. At the end of the treatment, the colonic tissues of mice were taken to detect the expression of NLRP3, ASC, Caspase-1, IL-18 and IL-1β in colon tissues by fluorescence quantitative RT-PCR, Elisa and immunohistochemistry respectively. Results: Compared with the model group, SASP group and medium and high dose Pulsatilla had significant therapeutic effect on IBD (P <0.01), but the therapeutic effect was inhibited in zVAD group (P <0.05). At the same time, middle and high doses of Pulsatilla could increase the expression of NLRP3, ASC, Caspase-1, IL-18 and IL-1β in colon tissues (P <0.05), while zVAD inhibited this upregulation (P <0.05). Conclusion: Pulsatilla Decoction may play an important role in the treatment of IBD by activating NLRP3 inflammasome and promoting the production of corresponding inflammatory factors including IL-18 and IL-1β.