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The channel based microfluidics, both continues flow and droplet flow, have been well established in Dalian Institute of Chemical Physics, and have got successful for the chromatography based separation and analytical chemistry/biochemistry.1, Continues flow: --3D micro-nanostructures chip --Wax patterned nitrocellulose (NC) paper and liquid mold based PDMS chip --Scale Integrated Immunoassay and DNA hybridizations --3D Tumor cells & microenvironment interaction (cell-cell, cell-mechanical force) --Valve-controlled intermittent immobilizing of individual C.elegans and Study of survival rate and lifespan.