【摘 要】
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Background, Motivation and Objective Optogenetics is a recently developed technique that has been widely implemented in the field of neuroscience. Channelrhodopsins (ChR), i.e., proteins that function
【机 构】
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Biomedical Engineering, Columbia University, New York, New York, USA
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Background, Motivation and Objective Optogenetics is a recently developed technique that has been widely implemented in the field of neuroscience. Channelrhodopsins (ChR), i.e., proteins that function as optically-activated ion channels, are introduced into neurons either by viral transduction or transgenic manipulations. Traditional viral transduction involves invasive direct infusion procedures which may pose unnecessary brain damage. Recent advances in focused ultrasound (FUS) offer an alternative, non-invasive approach to carrying out viral transduction in the brain as shown by our group and others. In the presence of microbubbles, the blood-brain barrier (BBB) can be temporarily opened, and we have demonstrated the feasibility of non-invasive AAV-GFP delivery to the targeted brain region. Taking advantage of the FUS-mediated BBB opening, we propose a FUS-based, non-invasive adenoviral delivery scheme suitable for optogenetic applications.
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