Time-resolved protein activation by proximal decaging in living systems

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  Precise perturbation of protein function with high selectivity and temporal resolution,especially in the gain-of-function manner,is crucial to dissect various dynamic biological processes.Although the chemical decaging of the active site has emerged as effective strategy for protein activation,only limited caged amino acids can be genetically encoded,as well as many enzymes do not have well-defined catalytic sites,rendering them incompatible with direct decaging.
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