Echinacea species (also known as coneflowers) are important plants in both pharmaceutical and ornamental markets.There are nine varieties of Echinacea plants but those most commonly used medicinally and in research are Echinacea angustifolia,E.pallida and E.purpurea for the prevention and treatment of common cold and wound healing.Currently,extracts and whole plants made from these three species comprise one of the largest sectors of the herbal medicine market in North America and in Europe.Moreover,purple coneflower (E.purpurea L.) is widely cultivated in gardens and grows in wild in some countries.Here,we reported the optimal conditions for callus induction and plant regeneration from in vitro explants ofE.purpurea; subsequently,we transformed the plasmid carrying a chimeric Petunia chalcone synthase construct by Agrobacteriurn-mediated method.The obtaining transgenic Echinacea plants were then characterized extensively.Chalcone synthase is a key enzyme in synthesizing anthocyanins/flavonoids,a large class of secondary metabolites.