【摘 要】
：
Objective Analysis the possible mechanism of PI-103, concomitant inhibition of PI3K and mTOR, improving the ovarian cancer SKOV3/DDP cells platinum sensitivity.Methods Cell proliferation test to test
【机 构】
：
Gynecology & Obstetrics Department,Zhong Da Hospital,Southeast University,Nanjing Jiangsu Province 2
【出 处】
：
中华医学会第十四次全国妇科肿瘤学术会议
论文部分内容阅读
Objective Analysis the possible mechanism of PI-103, concomitant inhibition of PI3K and mTOR, improving the ovarian cancer SKOV3/DDP cells platinum sensitivity.Methods Cell proliferation test to test ovarian cancer cells growth inhibition rate and ICS0 ; obtain the changes of cell cycle and cell apoptosis in ovarian cancer cells by flow cytometry instrument; Western blot analysis the expression of Cyclin D1, p21, cl-caspase 3, Akt,p-Akt, S6 and pS6.
其他文献
Objective: Survivin is an inhibitor of apoptotic proteins.In this study, the expression levels of survivin in laryngeal squamous carcinoma were detected and the effect of survivin on cell proliferatio
Objective: DNA methylation and histone modification are common epigenetic changes.The epigenetic change and CpG island methylator phenotype are closely related to poor prognosis of neuroblastoma (NB)
Objective: To examine the effect of dual blockage of EGFR and PI3K on radiosensitivity of breast cancer cells.Methods: The MCF-7 cell line, which had a lower expression of EGFR, was chosen as the nega
Objective: To determine whether biomarkers which can be identified in the plasma are predictive for response to EGFR-TKIs and patient survival time.Methods: Formalin-fixed and paraffin-embedded biopsi
Objective: To assess the feasibility for Rawals minilaparatomy radical cystoprostatectomy (RCP) and ileal conduit (IC).Methods: Four patients with muscle-invasive urinary bladder cancer underwent RCP
Objective: The effect of neoadjuvant chemotherapy (NAC)on the survival of patients with invasive bladder cancer remains controversial despite the randomized controlled trials (RCTs)of more than 3,000
Objective: To elucidate the clinical significance of ALKrearrangement in selected advanced NSCLC patients and to evaluate a possible association between ALK expression and clinical outcomes in ALK-pos
目的 现在已经有很多相关研究证实Fezl是一种抑癌基因,在多种肿瘤组织中表达较正常组织来说有不同程度的降低.在肿瘤细胞中将Fezl进行过表达之后能够使肿瘤细胞停留在G2/M期的细胞增加.本实验旨在研究Fezl基因在调控乳腺癌细胞系MCF-7生长与增殖中的作用.方法 构建Fezl-GFP质粒并将其与GFP质粒分别转染入MCF-7细胞中,通过流式细胞仪检测MCF-7细胞周期的变化.
目的 研究曲古抑素A (Trichostatin A,TSA)诱导肺癌细胞株A549细胞周期阻滞及细胞凋亡及其相互作用,并探讨其可能的作用机制.方法 采用MTT法检测不同浓度TSA对A549细胞的抑制作用,流式细胞仪检测不同浓度及时间段细胞凋亡的情况及细胞周期的变化.Western印迹检测处理后A549细胞H3乙酰化表达水平.加入Caspase抑制剂Z-VAD-FMK后检测TSA处理细胞的凋亡及周
目的 探究Twist介导的衰老逃逸在宫颈癌侵袭转移过程中的作用.方法 免疫组织化学检测93例正常宫颈上皮以及宫颈鳞状细胞癌标本中Twist和衰老指标CBX3的表达;合成靶向Twist的小干扰RNA瞬时转染宫颈鳞癌细胞SiHa,免疫细胞化学及β-半乳糖苷酶染色检测细胞衰老变化.结果 Twist在正常宫颈上皮、早期宫颈鳞癌(ⅠA～ⅡA期)及晚期宫颈鳞癌(ⅡB～ⅢB期)中强阳性表达率分别为4.3％、44