Agaricus blazei Murill (AbM), the edible Basidiomycetes mushroom, has been traditionally used as a health food source in Brazil for the prevention of cancer, diabetes, infection,arteriosclerosis and chronic hepatitis.In this study, BALB/c mice were fed with A.blazei M polysaccharides at different time points to investigate the anti-inflammatory action and the immunomodulatory effects.When mice were administrated AbM polysaccharides for a period of time and then injected LPS into mice, the Trem-1HI (Triggering receptor expressed on myeloid cell expression on macrophages is inhibited.The expressions of CD40HI and CD8αHI markers on DCs increased apparently.However CD8HI maker on macrophages decreased obviousiy, this result probably regulated by the activation of CTL-4 (cytotoxic T lymphocyte antigen 4) and (ICOS inducible co-stimulator from T cells.In addition, the Trem-1 、 TNF-α、IFN-γ、 iNOS、 IL-1β、 IL-6、 IL-12、 CCL2 (Chemokine (C-C motif) ligand 2) and PGE2 (Prostaglandin E2) mRNA cytokines of mice were inhibited obviously after feeding with AbM detected by real-time PCR.All of the results demonstrated that AbM polysaccharides possesses effects on both immunomodulation and anti-inflammation.These markers may serve as immune targets for further understanding the molecular basis and efficacy of AbM and other Chinese herbal medicines in the future.