【摘 要】
：
Cysteine-rich secretory proteins(CRISPs),characterized by 16 conserved cysteines,are distributed in a wide range of organisms.In the previous studies,a novel CRISP family member(cysteine-rich buccal g
【机 构】
：
School of Life Sciences,Liaoning Normal University,Dalian,116081
【出 处】
：
中国生物化学与分子生物学会2016年全国学术会议
论文部分内容阅读
Cysteine-rich secretory proteins(CRISPs),characterized by 16 conserved cysteines,are distributed in a wide range of organisms.In the previous studies,a novel CRISP family member(cysteine-rich buccal gland protein,CRBGP)was separated from the buccal gland of lampreys(Lampetra japonica,L.japonica).Lamprey CRBGP could not only suppress depolarization-induced contraction of rat tail arterial smooth muscle,but also block voltage-gated sodium channels(VGSCs).In the present study,the anti-angiogenic activities of CRBGP were investigated using endothelial cells and chick chorioallantoic membrane(CAM)models.In vitro assays,CRBGP is able to induce human umbilical vein endothelial cells(HUVECs)apoptosis by disturbing the calcium homeostasis and mitochondria functions.In addition,CRBGP could inhibit proliferation,adhesion,migration,invasion and tube formation of HUVECs by affecting the organization of F-actin and expression level of matrix metallo-proteinase 2(MMP-2),matrix metallo-proteinase 9(MMP-9)and vascular endothelial growth factor A(VEGFA)which are related to angiogenesis.In vivo assays,CRBGP could suppress the blood vessel formation in CAM models.Therefore,CRBGP is an important protein present in the buccal gland of lampreys and might help lampreys suppress the contraction of blood vessels,nociceptive responses and wound healing of host fishes during their feeding time.
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