牛贝氏贝诺孢子虫巢式PCR检测方法的建立

来源 :中国畜牧兽医学会兽医寄生虫学分会第十三次学术研讨会 | 被引量 : 0次 | 上传用户：lyl_chong

【摘要】

：

根据GenBank上发表的贝氏贝诺孢子虫核糖体内转录间隔区(ITS)序列,在传统PCR方法的基础上设计一对特异性内引物,建立贝氏贝诺孢子虫巢氏PCR检测方法并进行了临床样品检测.结果表明,建立的巢式PCR检测方法能检测到贝氏贝诺孢子虫DNA的最低浓度为24.3 ag/μL,检测刚地弓形虫、犬新孢子虫、牛巴贝斯虫、环形泰勒虫、边缘无浆体等病原体均为阴性,196份临床检测样本发现,3份为阳性,阳性率为

【作者】

：

王文涛付雪岳东梅宿欣常巧呈张艳王春仁

【机构】

：

黑龙江八一农垦大学动物科技学院,黑龙江大庆,163319;东北农业大学,黑龙江哈尔滨,150030

【出处】

：

中国畜牧兽医学会兽医寄生虫学分会第十三次学术研讨会

【发表日期】

：

2015年6期

【关键词】

：

贝氏贝诺孢子虫巢氏PCR 检测核糖体内转录间隔区

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牛贝氏贝诺孢子虫巢式PCR检测方法的建立

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