【摘 要】
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Objective: This project constructed the lentivirus over-expression vector of mice C-X-C chemokine receptor type4(Cxcr4) gene based on previous research, conducted in vitro transfection of mice mesench
【机 构】
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Affiliated Hospital of Nanjing University of Chinese Medicine, 155 Hanzhong Road, Nanjing 210029, Ch
【出 处】
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第十四次全国中西医结合防治呼吸系统疾病学术研讨会
论文部分内容阅读
Objective: This project constructed the lentivirus over-expression vector of mice C-X-C chemokine receptor type4(Cxcr4) gene based on previous research, conducted in vitro transfection of mice mesenchymal stem cell (MSCs), co-transplanted with hematopoietic stem cell (HSCs) acquired through magnetic activated cell sorting, and reconstructed the mice bone marrow chimeric models to explore the effects of over-expressed CXCR4 on MSCs homing, together with the influence on the recovery of the entire mice bone marrow hematopoietic system.
其他文献
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目的:桑色素已被发现在诸多疾病模型中具有良好的疗效,具有潜在的抗炎作用,然而在肺部疾病中尚未见相关报道.本研究旨在观察其对慢性哮喘小鼠气道炎症的作用并探讨相关机制.方法:雌性BALB/c小鼠随机分为正常对照组、哮喘组、桑色素低剂量和高剂量组、地塞米松组、溶剂对照组,OVA致敏激发建立慢性哮喘气道炎症模型,激发前以桑色素及地塞米松进行干预.各组小鼠予肺功能检测;取肺组织切片行病理染色;收集支气管肺泡
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目的:通过建立免疫低下肺部感染小鼠模型,探讨清肺培元颗粒对免疫低下肺部感染小鼠的炎症状态的影响.方法:将60只BALB/c雌性小鼠,随机分为六组,每组10只,即正常组、模型组、中西药组(清肺培元颗粒加盐酸左氧氟沙星,4.005g/kg/d)、清肺培元颗粒高、中、低剂量组(分别为4g/kg/d、2g/kg/d、1g/kg/d).除正常组外其他各组腹腔注射FLV病毒,21d后鼻腔滴注肺炎链球菌,建立免
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Objective: To investigate the prevalence and the potential risk factors of anxiety and depression among chronic respiratory diseases patients in general hospitals in Jiangsu Province of China.