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Eleven self-compatible B.napus accessions were obtained crossing with S-1300 whose F1 hybrids showing stable self-incompatibility,and had two recessive class Ⅱ S haplotypes as BnS-7(BrS-29)on A genome and BnS-6(BoS-15)on the C genome.Dominant functional BnSP11-1300 on A genome determines the self-incompatibility through S-1300 to its F1 hybrids while co-dominance with SRK expression.