Fusarium head blight (FHB) or scab caused by Fusarium graminearum is an economically important disease on small grain cereal crops worldwide and is especially serious in China.Over the past three decades, benzimidazole fungicides, particularly carbendazim, have been relied on to control FHB in China.However, repeated applications of these fungicides have caused the emergence of resistant strains leading to control failures.Previous studies show that resistance of F.graminearum to carbendazim is mainly caused by point mutations in the β2-tubulin gene.The results reveal that alterations either at amino acid codons 167 (F167Y) or 200 (F200Y) in β2-tubulin are linked to moderate carbendazim resistance.The proportion of resistance strains (F167Y) at codon 167 is up to 95%.Therefore, we developed a new loop-mediated isothermal amplification (LAMP) method for visual detection of F.graminearum (F167Y)with resistance to carbendazim.The LAMP reaction was optimal at 63 ℃ for 60 min.When hydroxynaphthol blue (HNB) was added before amplification, samples with F.graminearum (F167Y)DNA developed a characteristic sky blue colour but those without DNA or with the DNA of the wild-types did not.Results obtained with LAMP and HNB were confirmed when LAMP products were subjected to gel electrophoresis.Because the LAMP assay reported here is simple, rapid, sensitive, and specific, it should be valuable for the monitoring and controlling Fusarium head blight caused by F.graminearum.