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Objective：The purpose of this study is to compare the clinical results of two bundle ACL reconstruction respectively with four and eight strands of hamstring tendons. Method 76 patients with chronic ACL rupture were randomized separated in two groups for two bundle ACL reconstruction:In the first group the semitendinosus tendon was used to make two two-stranded grafts, In the second group the semitendinosus tendon was used to make one four-stranded graft, and the gracilis tendon was used to make the other four-stranded graft. 33 patients in the four-strands group and 35 patients in the eight--strands group were followed up for more than one year. IKDC and Lysholm scales were used toevaluate the clinical results. Results in the four-stranded graft group, the side to side difference was ＜3mm in 25 patients (75.8％), 3ram to 5mm in 5 patients (15.2％), 6 to lOmm in 3 patients (9.0％), pivotshift test was negative in 28 patients, and positive in 5 patients (15.2％); in the eight-stranded graft group, the side to side difference was ＜3mm in 33 patients (94.3％), 3mm to 5mm in 2 patients (5.7％);pivot shift test was negative in 34 patients, and was positive in 1 patients (2.9％). According to the IKDC scale, 29 patients (87.9％) in the four-strands group, and 34 patients (97.1％) in the eight-strands group were grade normal or nearly normal (p＜0.05). The Lysholm score were 90.2±2.9 and 97.3± 1.7(P＜0.05). Conclusion, arthroscopic two-bundle ACL reconstruction with eight strands of hamstring tendons is more reliable to restore the stability of the knee than four strands of hamstring tendons.