【摘 要】
：
Conventional transgenic and gene targeting approaches to generate the genetically modified mouse (GEM) models have their own advantages and disadvantages.Using DNA pronuclear injection to generate tra
【机 构】
：
Technology Cyagen Biosciences, Inc.USA/China
论文部分内容阅读
Conventional transgenic and gene targeting approaches to generate the genetically modified mouse (GEM) models have their own advantages and disadvantages.Using DNA pronuclear injection to generate transgenic models is relatively fast and cost effective, which usually produces the over expression of gene of interest with random integration and multiple copies.
其他文献
Lgr5 has been approved to be the stem cell marker in mouse gastrointestinal tract.Evidences also show that Lgr5+ and Bmi1+ cells may represent two stem cell sub-populations with different functions in
The esbablishrnent of human embryonic stem cells, and lately, induced pluripotent stem cells (hESC, hiPSC, respectively) greatly expanded our view to subtly analyze the cellular and molecular happenin
Clostridium difficile (Cd) is a well known emerging pathogen of opportunistic infection in hospitals worldwide and is the major causative antibiotic-associated pseudo-membranous colitis and diarrhea i
The development of the stem cell therapy has been recognized for solving various rare and incurable diseases that modem medicine has not solved yet.Over the past 10 years of hard work and investment i
Recent studies have revealed that following injuries, soft tissues such as anterior cruciate ligaments (ACL), release large amounts of matrix metalloproteinases (MMPs).These enzymes have a devastating
In bone tissue engineering and regeneration we have generated a unique transgenic mouse line, in which luciferase and GFP reporters are driven by a bone specific gene promoter and an actin promoter, r
Although induced pluripotent stem cells (iPSCs) can be reprogrammed from human somatic cells using four defined factors, the process is extremely inefficient, as there is a strong epigenetic block pre
Objective: To investigate the efficiency ofautologous transplantation of peripheral blood stem cells for treatment of patients with diabetic lower limb ischemia.Methods: Eighteen patients of type 2 di
Emerging evidence indicates that human mesenchymal stem cells (hMSCs) can be recruited to tumor sites, and affect the growth and development of human malignancies.However, there is some controversy re
Introduction: Young patients receiving chemotherapy occasionally face infertility and premature ovarian failure (POF).Numerous investigations reported that adipose-derived stem cells (ADSCs) transplan