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Recently, we have utilized micro/nanometer-scale bare silica capillaries to separate DNA molecules under pressure-driven flow conditions.After a fluorescence dye intercalated DNA sample was hydrodynamically injected, a pH-buffered solution was pressurized into the capillary, carrying the DNA molecules to an on-line laser-induced fluorescence detector.DNA of a wide size range (from 75 bp to 106 kb) was beautifully separated in a single run.The elution order followed the DNA size exactly, with the longest DNA being eluted out first and the shortest DNA being eluted out last.However, the separation mechanism was not clear.