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  5. High level expression of anti-TNF-α monoclonal antibody in CHO/DHFR-cells

High level expression of anti-TNF-α monoclonal antibody in CHO/DHFR-cells

来源 :The 12th Meeting of the Asia Pacific Federation of Pharmacol | 被引量 : 0次 | 上传用户：bobo82111
【摘 要】
：
Aim: TNF-α has been proved as a therapeutic target for rheumatoid arthritis.However, the cost of anti-TNF-α biologic agents is considerable and unacceptable at present.Here, a high level expression sy
【作 者】
：
Peng WANG Xue-gang LUO Chong-xi WANG Guang HU Tong-cun ZHANG 
【机 构】
：
Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Bioenginee
【出 处】
：
The 12th Meeting of the Asia Pacific Federation of Pharmacol
【发表日期】
：
2013年4期
【关键词】
：
rheumatoid arthritis anti-TNF-α mcAb CHO/DHFR-cell bicistronic expression 
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Aim: TNF-α has been proved as a therapeutic target for rheumatoid arthritis.However, the cost of anti-TNF-α biologic agents is considerable and unacceptable at present.Here, a high level expression system of anti-TNF-α monoclonal antibody (mcAb) was constructed in dihydrofolate reductase-negative Chinese hamster ovary (CHO/DHFR-) cells.
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