Comparison of Tolerogent Dendritic Cells Induced by Liver X Receptor Agonist from Bone Marrow-derive

来源 :第十三届亚洲泌尿外科学会年会(UAA2015)、第二十二届全国泌尿外科学术会议(CUA2015)、第十六届全军及武警泌尿 | 被引量 : 0次 | 上传用户:srepair555
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Previous studies indicated that tolerant dendritic cells (tDCs) could be induced by a liver X receptor (LXR) agonist from mouse bone marrow cells, but the characteristics and potential mechanism of these tDCs still need to be further explored.Mouse bone marrow cells were pulsed with interleukin (IL)-4 and granulocyte-macrophage colonystimulating factor (GM-CSF) with or without LXR agonist-T0901317 for 7 days or induced with lipopolysaccharide for 3 days.Then cellular biomarkers, T cell viability, and the ability to induce regulatory T cells (Treg) formation and cytokines in culture supernatants were tested.CD4+ T cells purified from BALB/c DO 11.10 RAG+ mice were used in a Treg induction assay.NF-κ B inhibitors and PI3K inhibitors were applied during these assays.Natural tDCs (N-tDCs) isolated from mouse spleens were used as controls.
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