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The fungi under genus Trichoderma are cosmopolitan in nature and widely used as biopesticides.Three hundred isolates of Trichoderma were isolated from stressed soils of arid and semi-arid regions of India.These isolates were screened in vitro for high temperature stress tolerance.Thirteen isolates with tolerance to lethal temperature of 50+C were identified.Among them, Trichoderma Iongibrachiatum 673 exhibited maximum tolerance levels when its conidial and hyphal structures were tested independently.