【摘 要】
：
目的 研究酿酒酵母突变株Y518高产谷胱甘肽的潜在机制,为代谢工程进一步构建谷胱甘肽高产菌株提供新的思路.方法 提取酵母总RNA进行高通量测序,并通过基于RNA高通量测序的转录组分析,筛选出与谷胱甘肽相关的差异表达基因.结果 转录组的统计数据分析表明共有1125个差异表达基因(fold-change＞=2.0,FDR＜=0.001)上调,503个下调.其中13个基因富集在谷胱甘肽代谢通路.γ-谷氨
【机 构】
：
常熟理工学院生物与食品工程学院,江苏常熟 215500
【出 处】
：
中国食品科学技术学会第十届年会暨第七届中美食品业高层论坛
论文部分内容阅读
目的 研究酿酒酵母突变株Y518高产谷胱甘肽的潜在机制,为代谢工程进一步构建谷胱甘肽高产菌株提供新的思路.方法 提取酵母总RNA进行高通量测序,并通过基于RNA高通量测序的转录组分析,筛选出与谷胱甘肽相关的差异表达基因.结果 转录组的统计数据分析表明共有1125个差异表达基因(fold-change＞=2.0,FDR＜=0.001)上调,503个下调.其中13个基因富集在谷胱甘肽代谢通路.γ-谷氨酰半胱氨酸合成酶是谷胱甘肽合成过程的限速酶,其编码基因GSH1显著上调；半胱氨酸是合成谷胱甘肽最重要的前体氨基酸,其合成酶的编码基因MET17也显著上调.此外,在氧化应激反应通路里,转录调节子编码基因SKN7及基因GPX2,CTT1,SOD1,TRX2也显著上调.生理学数据分析显示Y518胞内活性氧的水平要显著高于出发菌株.结论 Y518胞内谷胱甘肽积累量的增加主要与GSH1和MET17的上调有关,而GSH1的上调是在氧化应激的条件下受到上调的转录调节子编码基因SKN7调节的.
其他文献
为增加净水污泥之再利用价值及广度,增加净水污泥之再利用方法,落实本公司资源永续利用之政策。台水公司与中华民国轻质骨材协会合作,成功开发以净水污泥制作成新的环保绿建材—轻质骨材。研究成果显示,净水污泥制作之轻质骨材,其混凝土的抗压强度超过50 MPa,可用于建造如台北101的超高层建筑物或大型的桥梁,更重要的是,可使整个结构物至少降低1/4的重量,对于抑制地层下陷有很大之帮助,亦可提高结构物的耐震度
This paper is a continuation of our work on the theme of developing continuous flow reactors for the pharmaceutical industry.The motivation and advantages were discussed extensively in [1], and in sum
The influence of the design of dual-zone ZoneFlow type structured catalytic reactors (Fig.1) on their performance in natural gas steam reforming under commercial operating conditions is studied by mea
In this research, heterogeneous catalytic ozonation was used in a ceramic membrane system to investigate its effect on the formation of trihalomethanes (THMs), which are recognized as cancerous agents
Novel monolithic catalysts were prepared with Pd as the active component, porous hollow silica nanoparticles (PHSNs) as washcoat and monolithic cordierite as the monolithic ceramic substrate, as shown
It is well-known that the conventional supported catalyst powders usually suffer from a large flow resistance, and the poor heat transfer through the powders will easily lead to unacceptably non-unifo
The low-temperature-low-pressure methanol synthesis from syngas over CuO/ZnO/Al2O3-based catalysts is a mature and well-developed process and many large industrial plants are in operation.Due both to
The convection and diffusion processes in porous media are widely applied into chemical engineering, energy, environment and biology fields, such as chemical reaction in packed beds and mass transfer
Barium is considered as the most efficient promoter in Ru/MgO catalysts.It was found that Ba2+ could not be adsorbed directly on the surface of Mg(OH)2 having positive charge during preparation of sup
Hydrocarbon selective catalytic reduction of NOx (HC-SCR) is an attractive process to reduce emissions from diesel engines [1].Ag supported on Al2O3 is a promising catalytic system [2].Problems to sol