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Self-powered electrochemical biosensors based on enzymatic biofuel cells is a kind of novel electrochemical biosensors,which combines the advantages of the self-powered performance of enzymatic biofuel cells and the high selectivity of traditional electrochemical biosensors.Compared with traditional electrochemical biosensors,the enzymatic biofuel cells-based self-powered electrochemical biosensors displayed a series of advantages[1-3]:(1)simplified electronics because a potentiostat is not required;(2)improved construction and miniaturization due to the use of two electrodes instead of three;(3)improved stability since a reference electrode(often failure-prone)is not needed.Following this definition,we reported many interesting self-powered electrochemical biosensors based on enzymatic biofuel cells.