Nanozymes are nanomaterials with enzymatic activities.They have received great attention recently due to their wide applications and distinct advantages over natural enzymes, even to the conventional artificial enzymes.Herein, we developed an self-assembly approach to integrated nanozymes.By adaptively encapsulating catalytic guests within a zeolitic imidazolate framework (ZIF) through a one-pot synthesis under mild conditions.The as-prepared integrated nanozymes enabled one-step glucose detection through the cascade enzymatic reactions.The integrated nanozymes showed numerous merits, such as considerable high catalytic activity, enhanced stability, and recyclability.The integrated nanozymes were used to determine cerebral glucose in living rats brains.Most importantly, when further combined with microdialysis and microfluidic techniques, an integrative analytical platform was built for real time monitoring dynamic changes of striatum glucose in living brains.The current studies not only demonstrate new ways to design high-performance nanozymes, but also provide effective platform for biomedical applications.