The first step in writing a scientific manuscript involves constructing an outline to organize the main ideas in aLogical order. Begin the outline by preparing central-message sentences that summarize the key points of themanuscript before briefly describing research-related in formation，such as the methods and materials. Next,write the principal findings and results, followed by the implications and conclusions. Each key point shouldbe organized and separately arranged in either chronologic order, order of importance, or some other well-structured organizing scheme. The next step is to identify the critical references that pertain to each pointOnce your outline is coherent with a unifying theme, write the purpose and objective of your study and thenwrite the methods, carefully providing enough detailed information to replicate your study, including the statis-tical analyses. Then write the results based on the appropriate analysis and statistics for your findings.