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通过提取富硒女贞子多糖、蛋白质和齐墩果酸,采用MTT法测定三大类物质体外对山羊外周血淋巴细胞增殖活性的影响。方差分析的多重比较结果显示富硒女贞子多糖、蛋白质和齐墩果酸体外均具有促进ConA刺激山羊外周血淋巴细胞分裂增殖的作用,且富硒女贞子蛋白质组分、齐墩果酸组分的作用效果明显优于普通女贞子组,各相应浓度比较差异显著比例分别达到63.64%、40.00%。说明富硒女贞子能进一步提高山羊外周血淋巴细胞的增殖活性。
The effects of three kinds of substances on the proliferation activity of peripheral blood lymphocytes in goats were determined by MTT assay, and the polysaccharides, proteins and oleanolic acid of Se-enriched Ligustrum lucidum were extracted. Multiple comparisons of ANOVA results showed that polysaccharide, protein and oleanolic acid of Se-enriched Ligustrum lucidum could promote the proliferation of goat peripheral blood lymphocytes stimulated by ConA in vitro. The protein components of selenium-rich Ligustrum lucidum, oleanolic acid The effect of the components is obviously superior to that of ordinary Ligustrum lucidum, and the significant differences among the corresponding concentrations are 63.64% and 40.00% respectively. Se-enriched Ligustrum lucidum can further enhance the proliferation activity of goat peripheral blood lymphocytes.