论文部分内容阅读
Background Cryptosporidium spp.,Giardia duodenalis,and Enterocytozoon bieneusi are common gastrointestinal protists in humans and animals.Little information is available about these parasites in captive wildlife in central China.Methods Two hundred three fecal specimens from 80 wildlife species were collected and their genomic DNA extracted.Cryptosporidium were characterized with a DNA sequence analysis of the small subunit rRNA(SSU rRNA),the actin,and the 70-kDa heat shock protein(hsp70)genes.Giardia duodenalis was identified by analyzing the sequences of the triosephosphate isomerase(tpi),glutamate dehydrogenase(gdh),and β-giardin(bg)genes.Enterocytozoon bieneusi was genotyped with a sequence analysis of the internal transcribed spacer(ITS)region of the SSU rRNA gene.Results Cryptosporidiumfelis,C.baileyi,and avian genotype Ⅲ were identified in three animals(1.5%),the manul,red-crowned crane,and cockatiel.Giardia duodenalis was found in five animals(2.5%): assemblage B in a white-cheeked gibbon and beaver,and assemblage F in a Chinese leopard and Siberian tigers.Thirteen genotypes of E.bieneusi(seven previously reported genotypes and six new genotypes)were detected in 32 animals(15.8%)A phylogenetic analysis of E.bieneusi showed that five genotypes(three known and two new)clustered in group 1;three known genotypes clustered in group 2;one known genotype clustered in group 4;and the remaining four genotypes clustered in a new group.Conclusions Zoonotic Cryptosporidium spp.,G.duodenalis,and E.bieneusi are maintained in wildlife and transmitted between them.Zoonotic disease outbreaks of these infectious agents possibly originate in wildlife reservoirs.