【摘 要】
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Hepatitis B virus X protein (HBx) plays an important roles in the development of hepatocellular carcinoma (HCC).Emerging evidence has shown the association of aberrantly expressed miR-221 with cancer
【机 构】
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Department of Laboratory Medicine,the First Affiliated Hospital of Chongqing Medical University
【出 处】
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中华医学会第八次全国中青年检验医学学术会议
论文部分内容阅读
Hepatitis B virus X protein (HBx) plays an important roles in the development of hepatocellular carcinoma (HCC).Emerging evidence has shown the association of aberrantly expressed miR-221 with cancer development,however,little is known about its potential role in HBV-related HCC.In the present study,functional studies suggest that HBx leads to the promotion of cell proliferation and cell growth viability.Obviously overexpressed miR-221 was found in HBx-transfected cell compared with the mock counterparts.Suppression of miR-221 significantly inhibited HCC cell proliferation.Western blot analysis indicates that ERα is downregulated in HCC tissues and cell lines.Bioinformatic analysis combining with validation experiments identified ERα as a direct target of miR-221.Our study suggests that miR-221 modulates HCC cancer cell proliferation by suppressing ERα,functioning as a tumor promoter.Our data imply that miR-221 has potential as a miRNA-based therapeutic target for HBV-related HCC.
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