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Wheat straw was used as substrate for cellulose production by solid-state fermentation (SSF) and submerged fermentation (SmF) with a microbial community named WSD-5.The aim of this study was to compare SSF and SmF in terms of the degradation of the substrate and the product of enzymes.Alkali-treated wheat straw was ground into 0.1-1.0 cm size with a universal high-speed smashing machines (Model FW80, Taisite Instrument, China).WSD-5 was cultured in a 100 mL Erlenmeyer flasks with 40 mL solid Mandels medium and 0.5 g tnground wheat staw (6 cm size) at 30℃.After 7 days of incubation, the wheat straw covered with microorganisms was transferred into 30 mL sterile water and shaked at 120 rpm for 30 min to get the microorganisms suspension.For SSF, the culture was carried out by fermenting 10 g of wheat straw supplemented with 15 mL of concentrated Mandels medium (10 times mineral salts and 55.6 g/L pepton, Mandels and Webber, 1969), ralatve humidity was 60%.For SmF, the culture was performed at 3% of wheat straw for a final volume of 100 mL and was agitated at 120 rpm in a constant temperature incubator shaker (Model HZQ-F160, HDL Apparatus, China).All cultures, in sextuplicate for each treatment, were done in 500 mL Erlenmeyer flasks and were inoculated at 2 mL microorganisms suspension after sealed with tin foil paper and autoclaved at 121 ℃ for 20 min.The blank control, in triplicate for each treatment, was not inoculated.