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The overuse of pesticides and fertilizers in agricultural activities which exceeded a controlled limit has given a serious impact to the ecology system stability.The aim of this study was to analyze the frequency of the micronucleus (MN) in buccal cells of farmers who have been exposed to pesticides by using the MN assay.Buccal swabs were collected from the farmers in Cameron Highlands (n=84) using the wooden tongue depressors.The farmers were interviewed in order to obtain the demographic data using a structured questionnaire.Cytogenetic analysis was carried out using the Acridine Orange (AO) stain 0.0025% (w/v).The frequency of MN as biomarker for cytogenetic damage was determined using a fluorescence microscope.The results showed that the MN frequency of the farmers was significantly greater (p<0.05) than that of the controls.Besides that, in the aspect of socio-demographic factors (gender, smoking habit, alcohol consumption, age, educational level and BMI), MN frequency of the farmers was also significantly higher (p<0.05) than that of the control groups.Furthermore, it is also found that there was a significant difference in MN frequency for the factors such as durations of pesticides (p<0.01) and fertilizers (p<0.05)spraying, practice of Personal Protective Equipment (PPE) (p<0.05) and the practice of farmers while handling pesticides (p<0.05).This suggests that those factors may affect the cytogenetic abnormality.Furthermore, the positive correlations existed between the MN frequency and the duration of pesticides (p<0.01, r=0.297) and fertilizers (p<0.05, r=0.265)exposures.Hence, this present study demonstrated that exposure to the pesticides may increase the MN formation among the farmers, and prolonged pesticides use may cause an induction of cytogenetic abnormalities to humans.