Digital PCR is a method for DNA absolute quantification.In digital PCR,by statistical analysis,the absolute number of template molecules can be counted without errors from amplification bias or standard reference.[1][2] Digital PCR has been previously demonstrated on a multiwell plate and in a number of microfluidic formats,here we demonstrated a digital PCR platform based on hot embossing of plastic materials.We found a reliable method for bonding PMMA-COC by using superheated water,making the PCR platform simple and inexpensive.[3] The microstructures were hot embossed on PMMA,providing a simple and duplicable way to fabricate the device,and the thin COC cover keeps fluorescence detection in high performance.After treating the surface,diluted template molecules,primers and PCR mixtures were loaded into the chip.Mineral oil was used for partitioning the sample into many individual PCR reactions.[4] Since the chip thickness is low,and the volume of each micro-reactor pool is very small,each temperature cycle time is reduced.After polymerase chain reaction,the fluorescent dye embedded in DNA strands will be detected by a CCD.Based on the statistical analysis of the number of fluorescent chambers,it is possible to accurately and efficiently complete the traditional PCR amplification in a short time.The method presented here avoid using of traditional complex and expensive chips,greatly reduces the cost of digital PCR.