@@Microchip electrophoresis (MCE) is known as a further miniaturized version of capillary electrophoresis (CE), which has received as a powerful technique for the separation of various chemical species. It offers many advantages over conventional analytical technologies including high speed, low consumption of sample and buffer, easy integration, automatization and miniaturization. Up to now, laser induce fluorescence (LIF) is the most widely used detection scheme in MCE due to its high sensitivity. Previous MCE-LIF-based work is usually focused on off-line derivatiztaion, which are time-consuming and involves complexed liquid handing procedures. In order to maintain the advantages of MCE-based analysis, it is indispensable to employ on-chip fluorescence derivatization to the MCE-LIF detection system. In the past decade, the fluorescence tagging reagents such as o-phthaldialdehyde (OPA), naphthalene- 2,3-dicarboxaldehyde (NDA), 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-F) and dichlorotriazine fluorescein (DTAF) have been used to on-chip derivatization for LIF detection. However, the sensitivity obtained from these tagging reagents is usually unsatisfactory.